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Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

DOI :

10.3791/62214-v

February 15th, 2021

February 15th, 2021

3,334 Views

1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University

We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.

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