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Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography

DOI :

10.3791/4142-v

10:11 min

September 5th, 2012

September 5th, 2012

17,186 Views

1College of Pharmacy, Division of Pharmacology and Toxicology, The University of Texas at Austin

A method to determine the time course of ethanol concentration in the brains of rats during operant ethanol self-administration is described. Gas chromatography with flame ionization detection is used to quantify ethanol in the dialysate samples, because it has the sensitivity required for the small volumes that are generated.

Tags

Microdialysis

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