JoVE Logo
Faculty Resource Center

Sign In

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model

DOI :

10.3791/4181-v

December 18th, 2012

December 18th, 2012

18,419 Views

1Department of Medicine, Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, 2UCLA AIDS Institute, 3Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA, 4Department of Medical and Molecular Pharmacology, David Geffen School of Medicine at UCLA, 5Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA

The generation and characterization of tumor specific T cells using humanized mice is described here. Human thymic tissue and genetically modified human hematopoietic stem cells are transplanted into immunocompromised mice. This results in the reconstitution of an engineered human immune system allowing for in vivo examination of anti-tumor immune responses.

-- Views

Related Videos

article

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

article

Introducing Shear Stress in the Study of Bacterial Adhesion

article

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

article

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

article

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

article

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

article

Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature

article

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

article

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori

article

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved