September 8th, 2012
•CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclease activity.
Tags
Related Videos
Using Micro-Electro-Mechanical Systems (MEMS) to Develop Diagnostic Tools
Cancer Borealis Stomatogastric Nervous System Dissection
Gross Dissection of the Stomach of the Lobster, Homarus Americanus
Generation of Human CD40-activated B cells
Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
Fluorescence Activated Cell Sorting of Plant Protoplasts
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media
Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
The Use of Chemostats in Microbial Systems Biology
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved