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Fluorescence Activated Cell Sorting of Plant Protoplasts

DOI :

10.3791/1673-v

February 18th, 2010

February 18th, 2010

24,307 Views

1Center for Genomics and Systems Biology, Department of Biology, New York University

A method for isolating specific cell types from plant material is demonstrated. This technique employs transgenic marker lines expressing fluorescent proteins in particular cell types, cellular dissociation and Fluorescence Activated Cell Sorting. Additionally, a growth setup is established here that facilitates treatment of Arabidopsis thaliana seedlings prior to cell sorting.

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Fluorescence Activated Cell Sorting

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