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Generation of Myospheres From hESCs by Epigenetic Reprogramming

DOI :

10.3791/51243-v

9:32 min

June 21st, 2014

June 21st, 2014

7,489 Views

1Muscle Development and Regeneration Program, Sanford-Burnham Institute for Medical Research, 2IRCCS Fondazione Santa Lucia

Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.

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Generating Myospheres

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