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15.1K Views
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13:27 min
October 16th, 2009
DOI :
10.3791/1373-v
Chapters
0:00
Title
0:14
Introduction
1:46
Preparation of Feeder Cells
2:27
Preparation of PBMC for CD40- Stimulation
4:38
8:33
CD40- Stimulation
9:50
Subcultivation
12:20
Analysis of CD40- Activated B Cells
12:55
Conclusion
Summary
In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.
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