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Generation of Human CD40-activated B cells

DOI :

10.3791/1373-v

October 16th, 2009

October 16th, 2009

14,852 Views

1Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program, University Hospital of Cologne, Department I of Internal Medicine

In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.

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