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Examining BCL-2 Family Function with Large Unilamellar Vesicles Video (Video) | JoVE

DOI :

10.3791/4291-v

October 5th, 2012

October 5th, 2012

7,698 Views

1Department of Oncological Sciences, Department of Dermatology, The Tisch Cancer Institute, The Graduate School of Biological Sciences, Mount Sinai School of Medicine

Biochemically-defined large unilamellar vesicles (LUVs) are a convenient model system to analyze BCL-2 family interactions with immediate implications in better understanding the mitochondrial pathway of apoptosis. A method to produce LUVs, along with standard BCL-2 family protein combinations and controls to examine LUV permeabilization, are presented.

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