Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
19.0K Views
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11:42 min
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January 22nd, 2015
DOI :
January 22nd, 2015
•1Institut Curie, Centre de Recherche, CNRS, UMR 168, PhysicoChimie Curie, Université Pierre et Marie Curie, 2Kavli Institute for Brain and Mind, University of California, San Diego, 3Molecular Physiology and Biophysics Section, National Institute for Neurological Disorders and Stroke, National Institute of Health
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Chapters in this video
0:05
Title
1:44
Giant Unilamellar Vesicle Growth by Electroformation
4:29
Giant Unilamellar Vesicle Growth by Gel-assisted Swelling
6:07
Harvesting and Transferring GUVs
7:33
Patch-clamping GUVs
9:14
Results: Microscopy and Patch Clamp Studies of KvAP GUVs
11:03
Conclusion
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