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Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation

DOI :

10.3791/50007-v

11:39 min

June 1st, 2013

June 1st, 2013

13,277 Views

1Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center , 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center

We describe the preparation of thin retinal slices from aquatic tiger salamanders (Ambystoma tigrinum) and explain how we use these slices to study synaptic processing in the retina by obtaining dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells.

Tags

Whole cell Recordings

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