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Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

DOI :

10.3791/50059-v

December 6th, 2012

December 6th, 2012

23,460 Views

1McGowan Institute for Regenerative Medicine, 2Department of Bioengineering, University of Pittsburgh, 3Department of Cardiothoracic Surgery, Children's Hospital of Pittsburgh of UPMC, 4Department of Surgery, University of Pittsburgh

A method to rapidly and completely remove cellular components from an intact porcine heart through retrograde perfusion is described. This method yields a site specific cardiac extracellular matrix scaffold which has the potential for use in multiple clinical applications.

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