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Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

DOI :

10.3791/58123-v

November 26th, 2018

November 26th, 2018

10,816 Views

1Regenerative Medicine Research, Texas Heart Institute, 2Lifegift Organ Donation Center, 3Biomedical Engineering, Texas A&M University

This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow.

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