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Isolation and Decellularization of a Whole Porcine Pancreas

DOI :

10.3791/58302-v

9:08 min

October 10th, 2018

October 10th, 2018

8,741 Views

1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, 2The Transplant Institute, Sahlgrenska University Hospital

Tissue engineering of the whole pancreas is a challenge because of its exocrine and endocrine functions. We show a method for the dissection of an intact porcine pancreas and the process of successful decellularization by perfusion of detergents Triton X-100, sodium deoxycholate, and deoxyribonuclease.

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Isolation

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