JoVE Logo
Faculty Resource Center

Sign In

Stereotactic Injection of MicroRNA-expressing Lentiviruses to the Mouse Hippocampus CA1 Region and Assessment of the Behavioral Outcome

DOI :

10.3791/50170-v

June 10th, 2013

June 10th, 2013

21,948 Views

1Department of Biological Chemistry and The Edmond & Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem

MicroRNAs have significant roles in brain structure and function. Here we describe a method to enforce hippocampal miRNA over-expression using stereotactic injection of an engineered miRNA-expressing lentivirus. This approach can serve as a relatively rapid way to assess the in vivo effects of over-expressed miRNAs in specific brain regions.

-- Views

Related Videos

article

Behavioral Assessment of the Aging Mouse Vestibular System

article

Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors

article

Using a Classroom-Based Deese Roediger McDermott Paradigm to Assess the Effects of Imagery on False Memories

article

A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice

article

A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay

article

An Automated Method for Assessing Visual Acuity in Infants and Toddlers Using an Eye-Tracking System

article

Author Spotlight: Analysis of Ovarian Anatomy in Migratory Insects to Overcome Experimental Challenges

article

Author Spotlight: Developing Low-Tech Balance Assessment Methods for Broad-Spectrum Healthcare Applications

article

Author Spotlight: Accurately Assessing Thyroid Hormone-Driven Motor Alterations in Mouse

article

Author Spotlight: Overcoming Challenges in Drosophila Sleep Measurement Using DAM System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved