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Stereotactic Injection of MicroRNA-expressing Lentiviruses to the Mouse Hippocampus CA1 Region and Assessment of the Behavioral Outcome

DOI :

10.3791/50170-v

June 10th, 2013

June 10th, 2013

22,163 Views

1Department of Biological Chemistry and The Edmond & Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem

MicroRNAs have significant roles in brain structure and function. Here we describe a method to enforce hippocampal miRNA over-expression using stereotactic injection of an engineered miRNA-expressing lentivirus. This approach can serve as a relatively rapid way to assess the in vivo effects of over-expressed miRNAs in specific brain regions.

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