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Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

DOI :

10.3791/50227-v

June 21st, 2013

June 21st, 2013

21,621 Views

1Department of Physiology and Biophysics, University of Washington

Reconstituting functional membrane proteins into giant liposomes of defined composition is a powerful approach when combined with patch-clamp electrophysiology. However, conventional giant liposome production may be incompatible with protein stability. We describe protocols for producing giant liposomes from pure lipids or small liposomes containing ion channels.

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