JoVE Logo
Faculty Resource Center

Sign In

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

DOI :

10.3791/50227-v

June 21st, 2013

June 21st, 2013

21,796 Views

1Department of Physiology and Biophysics, University of Washington

Reconstituting functional membrane proteins into giant liposomes of defined composition is a powerful approach when combined with patch-clamp electrophysiology. However, conventional giant liposome production may be incompatible with protein stability. We describe protocols for producing giant liposomes from pure lipids or small liposomes containing ion channels.

Tags

Giant Liposome Preparation

-- Views

Related Videos

article

Methods for Patch Clamp Capacitance Recordings from the Calyx

article

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

article

Pressure-polishing Pipettes for Improved Patch-clamp Recording

article

Preparation of Artificial Bilayers for Electrophysiology Experiments

article

Spinal Cord Electrophysiology

article

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

article

Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography

article

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

article

One-channel Cell-attached Patch-clamp Recording

article

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved