JoVE Logo
Faculty Resource Center

Sign In

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

DOI :

10.3791/2269-v

January 10th, 2011

January 10th, 2011

20,617 Views

1Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, 2Department of Biomedical Engineering, Washington University in St. Louis, 3Department of Biomedical Engineering and Cardiac Bioelectricity and Arrhythmia Center, Washington University in St. Louis

Ionic current of BK channels is recorded using patch clamp techniques. BK channels are expressed in Xenopus oocytes by injecting messenger RNA. The intracellular solution during patch clamp recordings is controlled by a perfusion system.

Tags

Patch Clamp

-- Views

Related Videos

article

Methods for Patch Clamp Capacitance Recordings from the Calyx

article

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

article

Pressure-polishing Pipettes for Improved Patch-clamp Recording

article

Microinjection of Xenopus Laevis Oocytes

article

Visualizing RNA Localization in Xenopus Oocytes

article

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

article

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

article

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

article

One-channel Cell-attached Patch-clamp Recording

article

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved