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Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

DOI :

10.3791/50289-v

September 24th, 2013

September 24th, 2013

23,845 Views

1Cardiovascular Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Division of Cardiology, Sapienza University

Here we describe the isolation of adult mouse cardiomyoctyes using a Langendorff perfusion system. The resulting cells are Ca2+-tolerant, electrically quiescent and can be cultured and transfected with adeno- or lentiviruses to manipulate gene expression. Their functionality can also be analyzed using the MMSYS system and patch clamp techniques.

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