JoVE Logo
Faculty Resource Center

Sign In

Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice

DOI :

10.3791/54555-v

October 23rd, 2016

October 23rd, 2016

12,725 Views

1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, 2Department of Bioengineering, University of Colorado Anschutz Medical Campus, 3Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus

Methods are demonstrated for the isolation of sinoatrial node myocytes (SAMs) from adult mice for patch clamp electrophysiology or imaging studies. Isolated cells can be used directly or can be maintained in culture to permit expression of proteins of interest, such as genetically encoded reporters.

Tags

Isolation

-- Views

Related Videos

article

Primary Culture of Adult Rat Heart Myocytes

article

Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging

article

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

article

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

article

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

article

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

article

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

article

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

article

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

article

Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved