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Isolation and Physiological Analysis of Mouse Cardiomyocytes

DOI :

10.3791/51109-v

September 7th, 2014

September 7th, 2014

22,436 Views

1Department of Medicine, Vanderbilt University, 2Department of Cell and Developmental Biology, Vanderbilt University

Individual cardiomyocytes from wild type and mutant mice can be isolated from the heart in order to study their contractility and calcium transients. This allows characterization of the contribution of cellular dysfunction to heart dysfunction from any cause.

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Isolation

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