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Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

DOI :

10.3791/53912-v

June 20th, 2016

June 20th, 2016

7,519 Views

1Department of Anaesthesiology, Pharmacology, and Therapeutics, University of British Columbia, 2Department of Biomedical Sciences, Midwestern University

Currently, most available calcium indicators are used to quantify cytoplasmic calcium transients as indirect measures of calcium released from the sarcoplasmic reticulum in cultured smooth muscle cells. This protocol describes the use of a specific FRET-based indicator that allows direct measurement of calcium signals within the sarcoplasmic reticulum lumen.

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Keywords Calcium Fluctuations

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