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Isolation of Embryonic Ventricular Endothelial Cells

DOI :

10.3791/50463-v

July 20th, 2013

July 20th, 2013

9,182 Views

1McAllister Heart Institute, University of North Carolina at Chapel Hill

Primary cell culture is a useful technique for analyzing specific populations of cells, particularly from transgenic mouse embryos at specific developmental stages. Herein, embryonic ventricles are dissected and dissociated, and antibody-conjugated beads recognize and separate out the endothelial cells for further analysis.

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Isolation

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