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Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

DOI :

10.3791/50371-v

June 7th, 2013

June 7th, 2013

17,410 Views

1Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich

We report an efficient and simple method to isolate embryos at early stages of development from Arabidopsis thaliana seeds. Up to 40 embryos can be isolated in 1 hr to 4 hr, depending on the downstream application. The procedure is suitable for transcriptome, DNA methylation, reporter gene expression, immunostaining and fluorescence in situ hybridization analyses.

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