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Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

DOI :

10.3791/50386-v

June 24th, 2013

June 24th, 2013

13,718 Views

1Department of Structural Biology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

We describe a correlative microscopy method that combines high-speed 3D live-cell fluorescent light microscopy and high-resolution cryo-electron tomography. We demonstrate the capability of the correlative method by imaging dynamic, small HIV-1 particles interacting with host HeLa cells.

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