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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

DOI :

10.3791/63730-v

6:43 min

May 3rd, 2022

May 3rd, 2022

2,733 Views

1Department of Molecular Biophysics & Biochemistry, Yale University, 2Department of Physics, Yale University

We present a protocol for implementing interference-reflection microscopy and total-internal-reflection-fluorescence microscopy for the simultaneous imaging of dynamic microtubules and fluorescently labeled microtubule-associated proteins.

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Simultaneous Interference Reflection

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