JoVE Journal

Immunology and Infection

A subscription to JoVE is required to view this content.

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

We provide a reproducible method to induce type 1 diabetes (T1D) in mice within two weeks by the adoptive transfer of islet antigen-specific, primary CD4+ T cells.

Chapters in this video

0:05

Title

1:11

Isolation of T Cells from Spleen and Lymph Nodes of BDC2.5 Mice

2:52

Fluorescence-activated Cell Sorting of Diabetogenic CD4+ T Cells from BDC2.5 Mice

4:28

Adoptive Transfer of Diabetogenic CD4+ T Cells from BDC2.5 Mice

5:12

Monitoring Recipient Mice for Hyperglycemia and T1D

5:36

Results: Adoptive T-cell Transfer

6:10

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More