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Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein

DOI :

10.3791/50390-v

May 31st, 2013

May 31st, 2013

10,922 Views

1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology

The DNA-binding protein from starved cells (Dps) plays a crucial role in combating bacterial stress. This article discusses the purification of E. coli Dps and the protocol for an in vitro assay demonstrating Dps-mediated protection of DNA from degradation by reactive oxygen species.

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