Defining the Kinetics of DSB Repair Complex Formation
3:38
Defining Nuclei in Immunofluorescence Images
5:44
Analyzing Localization to a Long-lasting Double-strand DNA Break
6:40
Results: Co-localization of Double-strand DNA Breaks and Repair Proteins by Immunofluorescence Microscopy
7:46
Conclusion
Transcript
This method can help answer key questions in the DNA repair field such as how DNA repair complex formation, localization, and resolution are regulated. The main advantage of this technique is that it can unambiguously detect changes and repair com
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Repair of double-strand DNA breaks is a dynamic process, requiring not only formation of repair complexes at the breaks, but also their resolution after the lesion is addressed. Here, we use immunofluorescence microscopy for transient and long-lasting double-stranded breaks as a tool to dissect this genome maintenance mechanism.