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Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS

DOI :

10.3791/50456-v

July 18th, 2013

July 18th, 2013

12,631 Views

1Department of Chemical Engineering, Massachusetts Institute of Technology

We present a simple protocol to obtain fluorescence microscopy movies of growing yeast cells, and a GUI-based software package to extract single-cell time series data. The analysis includes automated lineage and division time assignment integrated with visual inspection and manual curation of tracked data.

Tags

Keywords Fluorescence Time lapse Microscopy

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