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Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

DOI :

10.3791/1863-v

March 24th, 2010

March 24th, 2010

10,637 Views

1Department of Biological Sciences and Purdue Center for Cancer Research, Purdue University

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae.

Tags

Morphological Phenotype

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