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4D Microscopy of Yeast

DOI :

10.3791/58618-v

April 28th, 2019

April 28th, 2019

8,001 Views

1Department of Molecular Genetics and Cell Biology, University of Chicago

This protocol describes the analysis of fluorescently labeled intracellular compartments in budding yeast using multi-color 4D (time-lapse 3D) confocal microscopy. The imaging parameters are chosen to capture adequate signals while limiting photodamage. Custom ImageJ plugins allow labeled structures to be tracked and quantitatively analyzed.

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