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Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

DOI :

10.3791/50490-v

January 17th, 2014

January 17th, 2014

11,622 Views

1Department of Pathology and Molecule Medicine, McMaster University

Colonization of the murine nasopharynx with Streptococcus pneumoniae and the subsequent extraction of adherent or recruited cells is described. This technique involves flushing the nasopharynx and collection of the fluid through the nares and is adaptable for various readouts, including differential cell quantification and analysis of mRNA expression in situ.

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