Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

14.7K Views

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10:32 min

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March 23rd, 2014

DOI :

10.3791/50620-v

March 23rd, 2014

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Ana Paula Fávaro Trombone¹, Sílvia Cristina Barbosa Pedrini², Suzana Madeira Diório³, Andréa de Faria Fernandes Belone¹, Luciana Raquel Vicenzi Fachin¹, Dejair Caitano do Nascimento⁴, Patricia Sammarco Rosa²

¹Department of Pathology, Instituto Lauro de Souza Lima (ILSL), ²Laboratory Animal House, Instituto Lauro de Souza Lima (ILSL), ³Department of Microbiology, Instituto Lauro de Souza Lima (ILSL), ⁴Department of Pharmacology, Instituto Lauro de Souza Lima (ILSL)

Summary

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Mycobacterium Leprae

Athymic Nude Mice

Bacilli Purification

Ziehl Neelsen Staining

Bacillary Counting

Frozen Stock Preservation

Strain Maintenance

Chapters in this video

0:00

Title

0:20

Preparation of Mycobacterium leprae Suspension

4:24

Cold Ziehl-Neelsen Stain

6:06

Viability Determination

7:48

Freezing/Thawing M. leprae Suspension

9:08

Mouse Inoculation

9:35

Results

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