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Isolation and Activation of Murine Lymphocytes

DOI :

10.3791/54596-v

October 30th, 2016

October 30th, 2016

20,457 Views

1School of Biological Sciences, College of Science, Nanyang Technological University

Lymphocytes are the major players in adaptive immune responses. Here, we present a lymphocyte purification protocol to determine the physiological functions of the desired molecules in lymphocyte activation in vitro and in vivo. The described experimental procedures are suitable for comparing functional capacities between control and genetically modified lymphocytes.

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Isolation

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