JoVE Logo
Faculty Resource Center

Sign In

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

DOI :

10.3791/50632-v

July 10th, 2013

July 10th, 2013

14,846 Views

1Department of Health Science & Technology, Cartilage Engineering & Regeneration, 2Biomaterials Department, Innovent e.V.

A bioprinter was used to create patterned hydrogels based on a sacrificial mold. The poloxamer mold was backfilled with a second hydrogel and then eluted, leaving voids which were filled with a third hydrogel. This method uses fast elution and good printability of poloxamer to generate complex architectures from biopolymers.

-- Views

Related Videos

article

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

article

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

article

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori

article

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

article

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

article

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

article

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

article

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

article

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

article

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved