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Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

DOI :

10.3791/54462-v

10:45 min

September 29th, 2016

September 29th, 2016

12,459 Views

1Department of Chemical and Biomolecular Engineering, University of Delaware, 2Department of Materials Science and Engineering, University of Delaware

We describe a sequential process for light-mediated formation and subsequent biochemical patterning of synthetic hydrogel matrices for three-dimensional cell culture applications. The construction and modification of hydrogels with cytocompatible photoclick chemistry is demonstrated. Additionally, facile techniques to quantify and observe patterns and determine cell viability within these hydrogels are presented.

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Light mediated Formation

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