JoVE Logo
Faculty Resource Center

Sign In

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

DOI :

10.3791/50736-v

September 27th, 2013

September 27th, 2013

10,866 Views

1Department of Orthopaedic Surgery, Stanford University , 2Department of Bioengineering, Stanford University

We describe the method of programming stem cells to overexpress therapeutic factors for angiogenesis using biodegradable polymeric nanoparticles. Processes described include polymer synthesis, transfecting adipose-derived stem cells in vitro, and validating the efficacy of programmed stem cells to promote angiogenesis in a murine hindlimb ischemia model.

-- Views

Related Videos

article

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

article

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

article

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

article

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

article

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

article

Polymeric Microneedle Array Fabrication by Photolithography

article

Fabricating Superhydrophobic Polymeric Materials for Biomedical Applications

article

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

article

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

article

Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved