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Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

DOI :

10.3791/50818-v

November 5th, 2013

November 5th, 2013

14,384 Views

1Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington

Confocal scanning microscopy is applied for imaging single mitochondrial events in perfused heart or skeletal muscles in live animal. Real-time monitoring of single mitochondrial processes such as superoxide flashes and membrane potential fluctuations enables the evaluation of mitochondrial function in a physiologically relevant context and during pathological perturbations.

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