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Single Cell Electroporation in vivo within the Intact Developing Brain

DOI :

10.3791/705-v

July 11th, 2008

July 11th, 2008

13,094 Views

1Brain Research Centre, University of British Columbia - UBC, 2Department of Cellular and Physiological Sciences, University of British Columbia - UBC

Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis tadpole.

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Single cell Electroporation

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