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Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors Video (Video) | JoVE

DOI :

10.3791/50833-v

December 3rd, 2013

December 3rd, 2013

6,896 Views

1INSERM UMR 1043, CNRS UMR 5282, Université Toulouse 3, 2UMR Viroligie, INRA ENVA ANSES

These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.

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