This protocol outlines the techniques used to produce Newcastle Disease Virus so that it can be administered safely to mice and other animals such as hamsters and sheep. The use of tangential flow filtration allows for large starting volumes to be
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Here we provide a detailed procedure for production, purification, and quantification of high-titer recombinant Newcastle disease virus. This protocol consistently yields > 6 × 109 plaque-forming units/mL, providing virus quantities appropriate for in vivo animal studies. Additional quality control assays to ensure safety in vivo are described.