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An Efficient Method for Adenovirus Production

DOI :

10.3791/61691-v

10:06 min

June 10th, 2021

June 10th, 2021

10,323 Views

1Institute of Cellular Biology and Pathology "Nicolae Simionescu"

Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.

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Adenovirus Production

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