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Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

DOI :

10.3791/50881-v

7:48 min

October 9th, 2013

October 9th, 2013

20,152 Views

1Department of Hematology and Oncology, University of Göttingen, 2Institute of Neuropathology, University of Göttingen, 3Institute of Anatomy and Cellbiology, University of Halle

The organotypic brain slice coculture with carcinoma cells enables visualizing morphological changes by fluorescence as well as bright field (video) microscopy during the process of carcinoma cell invasion of brain tissue. This model system also allows for cell exchange and replenishment approaches and offers a wide variety of manipulations and analyses.

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Keywords Coculture

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