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Single-plant, Sterile Microcosms for Nodulation and Growth of the Legume Plant Medicago truncatula with the Rhizobial Symbiont Sinorhizobium meliloti

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20:01 min

October 1st, 2013

October 1st, 2013

16,823 Views

0:05

Title

2:37

Preparation of M. truncatula A17 Seedlings: Sterilization of Seeds

5:01

Preparation of M. truncatula A17 Seedlings: Germination

7:00

Preparation of Individual Plate Microcosms

8:29

Laying out M. truncatula Seedlings on the Individual Microcosms

9:59

Inoculation and Sealing of Microcosms

12:10

Examination of Root Systems and Quantitation of Symbiotic Phenotypes

13:02

Results: Symbiotic Growth of M. truncatula and S. meliloti in Sterile, Single-plant Microcosms

19:03

Conclusion

Growth of Medicago truncatula plants in symbiosis with the nitrogen-fixing bacteria Sinorhizobium meliloti in individual, sterile microcosms made from standard laboratory plates permits frequent examination of root systems and nodules without compromising sterility. Plants can be maintained in these growth chambers for up to 9 weeks.

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