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An Improved Method for the Preparation of Type I Collagen From Skin

DOI :

10.3791/51011-v

January 21st, 2014

January 21st, 2014

22,338 Views

1Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital, 2Department of Anesthesia, Harvard Medical School

Traditional procedures for the isolation of soluble type 1 collagen (COL1) require about 10 days from start to finish because of lengthy buffer incubations and laborious resuspensions of fibrils. Here, we describe a means to purify COL1 from small dermal biopsies in less than 3 hr.

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Type I Collagen

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