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Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

DOI :

10.3791/52589-v

April 20th, 2016

April 20th, 2016

17,373 Views

1Department of Biotechnology and Biosciences, University of Milano-Bicocca, 2Boston Children's Hospital, Division of Gastroenterology, Harvard Medical School, 3Humanitas Clinical and Research Center

The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

Tags

Single cell Suspensions

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