January 8th, 2014
•Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.
Tags
Related Videos
Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
Synthesis of an Intein-mediated Artificial Protein Hydrogel
Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
Biomechanical Characterization of Human Soft Tissues Using Indentation and Tensile Testing
Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved