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Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

DOI :

10.3791/51055-v

October 27th, 2013

October 27th, 2013

14,242 Views

1Institute of Microbiology, University Hospital Center and University of Lausanne

Amoebal coculture is a cell culture system using adherent amoebae to selectively grow intracellular pathogens able to resist phagocytic cells such as amoebae and macrophages. It thus represents a key tool to discover new infectious agents. Amoebal enrichment allows discovery of new amoebal species and of their specific intracellular bacteria.

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