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Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

DOI :

10.3791/57766-v

September 27th, 2018

September 27th, 2018

8,316 Views

12I, UVSQ, INSERM UMR1173, Université Paris-Saclay, 2Institut Pasteur, Unité de Pathogénomique Mycobactérienne

Here, we present two protocols to study Phagocyte-Mycobacterium abscessus interactions: the screening of a transposon mutant library for bacterial intracellular deficiency and the determination of bacterial intracellular transcriptome from RNA sequencing. Both approaches provide insight into the genomic advantages and transcriptomic adaptations enhancing intracellular bacteria fitness.

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