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Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

DOI :

10.3791/50300-v

April 2nd, 2013

April 2nd, 2013

10,326 Views

1Department of Molecular Microbiology & Immunology, Oregon Health & Science University

This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.

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