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Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

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08:20 min

December 20th, 2013

December 20th, 2013


0:05

Title

1:24

Preparation of Solutions and Embryos

3:17

Immunofluorescence Staining and Microscopy

5:43

Representative Results

7:56

Conclusion

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

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